Q&A: HOW CAN ADVANCES IN TISSUE CLEARING AND OPTOGENETICS CONTRIBUTE TO OUR UNDERSTANDING OF NORMAL AND DISEASED BIOLOGY?

Q&A: How can advances in tissue clearing and optogenetics contribute to our understanding of normal and diseased biology?

Abstract Mammalian organs Sponge Soap comprise a variety of cells that interact with each other and have distinct biological roles.Access to evaluate and perturb intact biological systems at the cellular and molecular levels is essential to fully understand their functioning in normal and diseased conditions, yet technical limitations have constrai

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Biocontrol Potential of Fungal Endophytes against Fusarium oxysporum f. sp. cucumerinum Causing Wilt in Cucumber

Endophytic Chofa fungi have received much attention as plant growth promoters as well as biological control agents against many plant pathogens.In this study, 30 endophytic fungal species, isolated from various plants in China, were evaluated using in vitro dual culture assay against Fusarium oxysporum f.sp.cucumerinum, causing wilt in cucumber.The

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Single cell electroporation for longitudinal imaging of synaptic structure and function in the adult mouse neocortex in vivo

Longitudinal imaging studies of neuronal structures in vivo have revealed rich dynamics in dendritic spines and axonal boutons.Spines and boutons are considered to be proxies for synapses.This implies that synapses display similar dynamics.However, spines and boutons do not always bear synapses, some may contain more than one, and dendritic shaft s

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